A live human breast cancer tissue, growing in a culture dish and expressing histone H2B (bound to DNA) tagged to green fluorescent protein (GFP) was photobleached with a 405 nm UV-laser. If the bleached protein is mobile in the tissue, the fluorescent signal is restored in a certain time. This novel method (Fluorescence Recovery After Photobleaching, FRAP) is used in the NanoL project to study the kinetics of repair of nanolesions in the human DNA. Here we produced lesions in three sectors of a circle and three different color channels were merged at the fluorescence microscope to reproduce the colors of the Beilstein Stiftung logo. Bar is 10µm and the whole picture is 93 x 70 µm. Image produced by the NanoBIC PhD student Maren Herrlitz, with the support of Rayees Khan and Frank Tobias at the GSI Helmholtzzentrum für Schwerionenforschung, Darmstadt.
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Druckversion: 14. November 2011, 16:07